雄激素受体RNA干扰质粒的构建及其对毛乳头细胞雄激素受体表达的抑制作用研究
| 作 者：储小燕, 范卫新, 许文嵘 |
| 单 位：南京医科大学第一附属医院皮肤性病科 |
| 基金项目：国家自然科学基金 |
| 摘 要： |
| 目的：构建雄激素受体（androgen receptor, AR）RNA干扰的真核表达质粒，并将其转染人毛乳头细胞（dermal papilllia cell，DPC）后观测其对AR表达的影响。方法：应用基因重组技术构建AR的 RNA干扰质粒，经测序鉴定后以脂质体转染方法将质粒转染DPC，Western blot 检测被转染细胞AR 蛋白的表达。结果：成功构建了雄激素受体RNA干扰的真核表达质粒，将其转染了人DPC后，经Western blot 证实AR RNA干扰质粒能使DPC细胞AR 蛋白含量显著下降。结论：AR的RNA干扰质粒能抑制DPC内AR的表达，该实验为雄激素性脱发的基因治疗的进一步研究提供了必要的基础。 |
| 关键词：雄激素受体；RNA干扰；雄激素性脱发；毛乳头细胞 |
Construction of RNA interference expression vector targeting to androgen receptor(AR) gene an its effect on the expression of androgen receptor in human dermal papilllia cell
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| Abstract: |
| Objective: To construct RNA interference(RNAi) expression vector targeting to androgen receptor(AR) gene and investigate whether the recombined plasmids could regulate the expression of AR in human dermal papillia cell(DPC). Methods: Three AR-specific siRNAs were selected and RNAi expression vectors were constructed, then the DPCs were transfected with the recombined plasmids by lipofectin. AR protein expression was detected by western blot. Results: (1) The eukaryotic expression vector Ri-AR were constructed and transfected DPCs successfully. (2) AR RNAi decreased the level of AR expression in DPCs. Conclusion: The results suggested that RNA interference could inhibit AR in DPCs, and it might be significant for the androgenic alopecia gene therapy. |
| Keywords: androgenic receptor; RNA interference; androgenic alopecia; DPC |
| 投稿时间：2009-01-06 |
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